Composite

Part:BBa_K3747139

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-21)


napEDABCGH Paracoccus denitrificans DSM 413

Nap is a dissimilatory, periplasmic nitrate reductase which is expressed under aerobic conditions and is required for aerobic denitrification [1]. Nap is the first enzyme in the denitrification pathway and reduces nitrate (NO3-) to nitrite (NO2-). To establish denitrification in Pseudomonas putida EM42, we expressed this construct and tested its activity to convert NO3- into NO2-.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3641
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3641
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 478
    Illegal NotI site found at 829
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3641
    Illegal BglII site found at 493
    Illegal BglII site found at 844
    Illegal BglII site found at 1792
    Illegal BamHI site found at 3040
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3641
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3641
    Illegal NgoMIV site found at 1037
    Illegal NgoMIV site found at 1507
    Illegal NgoMIV site found at 2865
    Illegal NgoMIV site found at 3071
    Illegal NgoMIV site found at 3091
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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